br Experimental Procedures br Author Contributions br Acknow

2018-10-20

Experimental Procedures Author Contributions Acknowledgments Introduction Induced pluripotent stem Givinostat (iPSCs) have similar properties as embryonic stem cells (ESCs), such as self-renewal and differentiation capacity (Park et al., 2008c; Takahashi and Yamanaka, 2006). Reprogrammi

br Results br Discussion Here

2018-10-20

Results Discussion Here, we demonstrate the generation of hESC reporter lines without the use of drug selection for both active and silent genes through the use of the iCRISPR system. Selection-free gene targeting eliminates the need for removal of drug-resistance cassette after identification

As was shown in this study along with many other

2018-10-20

As was shown in this study, along with many other previous studies, TALEN and double-nicking CRISPR/Cas9 genome-editing techniques are significantly accurate, with only a negligible risk of random gene disruption, in the drug-selected clones. This result indicates that disrupting a particular gene w

br Results br Discussion Frequent

2018-10-20

Results Discussion Frequent tumor relapse in multiple tumor types has now been attributed to the presence of residual CSCs after conventional treatments. We and others have previously identified CD133 to mark a liver CSC subpopulation in HCC (Ma et al., 2007, 2010). Yet, the functional paths b

Both natural and synthetic approaches have

2018-10-20

Both natural and synthetic approaches have been used to generate fully defined hPSC culture substrates. ECM proteins such as vitronectin and laminins 511 and 521 have been shown to maintain hESC pluripotency and self-renewal (Braam et al., 2008; Domogatskaya et al., 2012; Miyazaki et al., 2012; Rodi

Reduction of heterogeneity during reprogramming is critical

2018-10-20

Reduction of heterogeneity during reprogramming is critical for revealing molecular roadmaps and precise mechanistic analysis. In this regard, the use of 2A peptides constituted a significant improvement (Carey et al., 2009; Kaji et al., 2009; Okita et al., 2008; Sommer et al., 2009; Yusa et al., 20

br Results br Discussion The pluripotency status of ES cells

2018-10-20

Results Discussion The pluripotency status of ES A-1210477 or iPS cells is maintained by a tightly regulated transcription factor network (Boyer et al., 2005). OCT4 is at the center of this network (Pardo et al., 2010; van den Berg et al., 2010) and is associated with chromatin modifiers to ma

The function of LRRK remains to be fully elucidated

2018-10-20

The function of LRRK2 remains to be fully elucidated, but studies have demonstrated a role for LRRK2 in neurite elongation and arborization. Cultured dopaminergic neurons from human LRRK2-G2019S-expressing transgenic mice and patient-specific induced pluripotent stem dopamine-2 receptor antagonist

Researchers are usually aware of the tissue that was

2018-10-20

Researchers are usually aware of the tissue that was initially used for isolation of MSCs, but there is evidence that accidental interchange of samples or contaminations with other 4-ethylphenyl sulfate can occur (Garcia et al., 2010; Torsvik et al., 2010). For established cell lines, some contamina

Recently several studies have suggested that peripheral bloo

2018-10-20

Recently, several studies have suggested that peripheral blood mononuclear purchase SYN-117 (PBMCs) are a promising resource for generating iPSCs because most of these cells do not undergo TCR rearrangement (Loh et al., 2010; Mack et al., 2011; Seki et al., 2010). However, PBMCs are composed of sev

TPCA reversibly binds to IKK and prevents it

2018-10-20

TPCA-1 reversibly binds to IKKβ and prevents it from phosphorylating IκBα for degradation and thereby blocks nuclear translocation of NF-κB (Kondo et al., 2008). Our results demonstrate that TPCA-1 may inhibit the stimulatory action of TNF-α on NF-κB. TPCA-1 partially inhibits secretion of constitut

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2018-07-29